Biacore is a life science products company, based in sweden. The handbook also includes a system description, safety instructions, specifications and a brief description of the spr principle. Core facility will help you with obtaining free downloads of manuals, tutorials and software. Biacore t200 lower sensorgram compared to biacore t100 upper sensorgram when using low levels of immobilized gpcr. Biacore t100 instrument handbook br100647 edition ad. Biacore x100 is intended for research use only and should not be used for diagnostic purposes in any clinical or in vitro procedures.
The symbols o and represent open and closed valves respectively. Biacore was founded in 1984 under the name of pharmacia biosensor ab, by researchers from pharmacia, linkoping institute of technology and the swedish national defence research institute foa, now renamed the swedish defence research agency or foi. The biacore 3000 and biacore t200 instruments housed in the biosensor core facility room 611, health science facility ii are accessible to faculty, students, fellows and staff members at the university of maryland, baltimore. Ge healthcare labelfree interaction analysis biacore 8k. Cross new frontiers in labelfree interaction analysis with biacore t200 28979412 data file. Instrument cleaning state which of the procedures are carried out just before starting a. Biacore t100 the latest protein interaction analysis system from. Parallel setup maximizing operational efficiency biacore 8k is designed to maximize operational efficiency by combining rapid highquality data with the smooth operation. This handbook describes in detail how to use the control and evaluation software. Click dock chip to dock the chip on to ifc or choose tools. Biacore t200 is a versatile system for highquality characterization of molecular interactions, from ions to viruses. Cytiva formerly ge healthcare life sciences 3,909 views.
Each angle of the reflected beam strikes the instrument s detector at a different point and, thus, the detector continuously records the position of reduced light intensity and calculates the spr angle from that figure. Biacore life sciences, a ge healthcare brand, is a leading global provider and manufacturer of laboratory and analytical equipment. Biacore sensorgram the systems also provide sensitive, accurate concentration measurements. Equipnet is constantly receiving used biacore equipment and analyzers, including the 2000 analyzer, 3000 analyzer, and others. The instrument also features a rack hotel in which up to 10 rack trays can be loaded with automatic rack exchange. Software wizard templates to help speed up assay optimization and routine analyses. The software provides dedicated support for kinetics, affinity. Biacore t100 software version 2 university of oxford. Use this handbook together with the specific user documentation for your biacore system, which will describe how to implement the assay principles in the instrument and system software. Insert a new chip and close the port cover by pressing the cover. Biacore offers high levels of expertise in their field, yielding high quality and reliable equipment for a number of lab and analytical needs. The biacore 4000 is ideal for laboratories that focus on drug discovery and antibody analysis with the ability to perform 60 hours of unattended operation, the biacore 4000 reduces labor by the researcher and increases productivity.
However, this checklist is also useful with other sensor chips. Biacore 8k is a highthroughput, highsensitivity spr system that enables efficient screening characterization, process optimization, and quality control of small molecules and biotherapeutics. Handbook of surface plasmon resonance richard b m schasfoort, richard schasfoort, arnoud marquart, peter schuck, erk t. The available sensor chip surfaces and immobilization techniques are. Oct 12, 2015 this video show you how to startup and prepare a biacore x100 system for use. The use of optical biosensors for studying macromolecular interactions is gaining increasing popularity. Biacore t100 instrument handbook describes the instrumentation in the biacore t100 system, with instructions for operation, maintenance and troubleshooting. Evaluation of the biacore 2000 instrument for screening of. Legal information regarding software licensing and details for acquiring additional licenses. Andrei zhukov, heptares therapeutics ltd, welwyn garden city, uk. Biacore t100 instrument handbook instrument handbook 88 pages ika hs 260.
Biacore t100 nanobiophysics usc dana and david dornsife. Biacore equipment is well known for its precision and accuracy in performance. How to use sensor chip sa in biacore 3000 and biacore 2000. C both for compartment containing the sample as well as the chip, buffer degasser and buffer selector up to four buffers can be used.
Each angle of the reflected beam strikes the instruments detector at a different point and, thus, the detector continuously records the position of reduced light intensity and calculates the spr angle from that figure. System overview instrumentation in the biacore t100 system is described in full in the biacore t100 instrument handbook. Labelfree interaction analysis is of increasing importance for scientists in the academic, pharmaceutical, biotechnology and diagnostic markets. Ge healthcare biosciences corp 800 centennial avenue, p.
Spr experiment checklist checklist explanation this checklist is written with the cm5 sensor chip in mind. Biacore t200 instrument handbook, the instruction manual for operating the biacore t200 instrument. Handbooks please note, only handbooks related to systems covered by your registered product keys will be displayed here. Overview of biacore systems and their applications. To access this section, you need to be logged in and have a valid product key registered in your account details. Its technology is often used to measure not only binding affinities, but kinetic rate constants and thermodynamics as well. According to our registry, biacore instruments is capable of opening the files listed below. Ge healthcare biacore t100 handbook pdf download manualslib. The chip with two flow cells is being used and all the reagents are injected manually.
Biacore t200 getting started 28984098 edition ab 3 contents biacore t200 getting started. Biacore t200 instrument handbook describes the instrumentation in the biacore t200 system, with instructions for operation, maintenance and troubleshooting. Measuring proteinprotein interactions using biacore. Biacore analyzers come in many different styles and models, ensuring they provide ideal solutions for a number of consumers needs and more. Biacore t200 getting started 28984098 edition ab 5 biacore t200 getting started biacore t200 getting started introduction this getting started handbook is designed as a selfstudy guide to introduce you to the basic operations of biacoretm t200, biacore t200 control software and biacore t200 evaluation software. Start biacore t100 control software and wait till the temperature stabilized. Standby puts the instrument in standby mode, which maintains a 1ow buffer or water f1ow through the f1ow.
Biacore 8k cytiva, formerly ge healthcare life sciences. Any functionality that is added in optional addon modules is described in separate documentation. Safety information biacore x100 instrument contains mains voltage and handles liquids that may be hazardous. Binding affinity measurement of antibodies from crude. Data file 29050011 aa labelfree interaction analysis biacore t200 software v2.
Bio rad s3 laboratory equipment instruction manual pdf. Biacore t100 instrument handbook guides you through operation and maintenance of the biacore t100 system. In biacore instruments, monochromatic light in the shape of a wedge a broad distribution of incident angles is used. Normalization with 40% glycerol biacore 20003000 or 70%. It is possible that biacore instruments can convert between the listed formats as well, the applications manual can provide information about it. Biacore systems characterize molecules in terms of their.
The systems also provide sensitive, accurate concentration measurements. Data file 29050011 aa labelfree interaction analysis. Please follow the manual of your spr instrument to implement this protocol. You can use biacore t200 instrument, on which you can include the solvent correction process in your experiment, using the calibration solutions 18 in the attached file.
Box 27, piscataway, nj 0885527 usa ge healthcare europe gmbh munzinger strasse 5, d79111 freiburg, germany ge healthcare biosciences kk sanken bldg. In one study, 1514 papers that involved the application of biosensor data were identified for the year 2009 alone rich and myszka, j mol recognit 24. Biacore t200 gxp package 28977954 biacore t200 upgrade kit 28977954 biacore t200 gxp upgrade kit 28977964 biacore t200 software v3. This edition of the biacore x100 handbook applies to systems running software version 2. Biacore x ge healthcare is manual surface plasmon resonance based refractometer, appropriate for the introductory investigation of interactions with unknown properties. Biacore t200 getting started guide, which in conjunction with the getting started reagent kit and cm5 sensor chip, provides users a selfguided tutorial through the basic steps of a basic biacore experiment performed using aminecoupling chemistry.
The molecular detection limit is 150 da, determining kinetic rate constants in the range of k ass 10 3 10 6 m1 s1 and k diss 101 105 s1. Specificity of interaction on and off rates kinetics binding strength affinity biacore sensorgram. Biacore t100 instrument and evaluation software ge healthcare. Falt experimental design for kinetic analysis of proteinprotein. Wipe regularly with a soft damp tissue and, if necessary, a mild cleaning agent. This handbook describes biacore t200 control software and evaluation software, version 1. Biacore 8k provides sensitivity and stability that enables the differentiation of tight binders with dissociation rate constants, k d, down to 106 s1. This is for informational purposes only, we do not offer this as a service. Download instruction manual of bio rad s3 laboratory equipment for free or view it online on.
Here the various biacore instrument platforms and their primary uses, ranging from semiautomated systems designed for simple, flexible basic research to fully automated, highthroughput systems, and systems designed to function in regulated environments, are all highlighted. Biacore t200 instrument handbook biacore t200 software handbook. Biacore surface matrix effects on the binding kinetics and. Consultations to determine if its applications are appropriate for your studies can be obtained from the core. Data file 29050011 aa labelfree interaction analysis biacore. Biacore t100 surface plasmon resonance instrument note. The first biacore spr instrument was commercially released in 1990. This machine performs surface plasmon resonance spr measuring macromolecular binding in real time. Evaluation of the biacore 2000 instrument for screening of low molecular weight affinity ligands title swedish abstract an important stage in the development of new affinity media for protein purification is the screening of potential affinity ligands for binding to the target protein. Single solution for interaction analysis allowing ranking, kinetics, affinity, concentration, and relative potency. A buffer se1ector va1ve determines which buffer f1ows over the sensor surface.
With the availability of the four routinely used biosensor platforms in our laboratory the biacore t100, proteon xpr36, octet red384, and ibis mx96, we performed a headtohead comparison study using a panel of highaffinity mousederived monoclonal antibodies against human proprotein convertase substilisin kexin type 9 pcsk9. Ge healthcare labelfree interaction analysis biacore t200. Instrument cleaning like unclogflushdesorbsanitizeother. This is based on the ability of the biomolecule of interest to interact with a specific binding partner, and may therefore be more informative than generic measurement techniques total protein concentration for example. The biacore t100 surface plasmon resonance instrument is used to analyze biomolecular interactions using surface plasmon resonance technology spr. Ge biacore t200 getting started guide ligand analyte. Biacore t200 cytiva, formerly ge healthcare life sciences. Assay design and setup for flu vaccine analysis duration. We recommend to use biacore x for the complex interactions and initial screenings. Schematic illustration showing the flow paths during loop injection. Comparison of biosensor platforms in the evaluation of high. One platform for comprehensive characterization of molecular interactions in terms of kinetics, affinity, specificity, comparability, concentration, immunogenicity, and thermodynamics. This handbook describes biacore t100 control software and evaluation software, version 2.